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Rat Matrix Metalloproteinase 8 (MMP8) ELISA Kit

SKU:
RTDL00684
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O88766
Sensitivity:
33pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
CLG2,HNC,PMNL-CL,Collagenase 2,Neutrophil Collagenase,PMNL collagenase
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Rat Matrix Metalloproteinase 8 (MMP8) ELISA Kit

The Rat Matrix Metalloproteinase 8 (MMP8) ELISA Kit is specially designed for the accurate measurement of MMP8 levels in rat serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring precise and consistent results for various research applications.MMP8 is a key enzyme involved in extracellular matrix remodeling and tissue repair processes. Its dysregulation has been linked to various pathological conditions, including inflammatory diseases, cancer metastasis, and tissue damage.

Studying MMP8 levels can provide valuable insights into these conditions and aid in the development of potential therapeutic strategies.With its advanced technology and reliable performance, the Rat MMP8 ELISA Kit is an indispensable tool for researchers looking to explore the role of MMP8 in biological processes and disease pathogenesis.

Product Name:Rat Matrix Metalloproteinase 8 (MMP8) ELISA Kit
Product Code:RTDL00684
Size:96 Assays
Target:MMP8
Synonyms:CLG2, HNC, PMNL-CL, Collagenase 2, Neutrophil Collagenase, PMNL collagenase
Detection Method:Sandwich
Range:78.125-5,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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