null

Rat Matrix Metalloproteinase 12 (MMP12) ELISA Kit

SKU:
RTDL00679
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q63341
Sensitivity:
32pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
MME,HME,ME,Macrophage Metalloelastase,Macrophage Elastase
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat Matrix Metalloproteinase 12 (MMP12) ELISA Kit

The Rat Matrix Metalloproteinase 12 (MMP12) ELISA Kit is specifically designed for the precise measurement of MMP12 levels in rat samples including serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring accurate and consistent results for various research applications.MMP12, also known as macrophage elastase, plays a critical role in tissue remodeling and inflammation processes. Dysregulation of MMP12 has been associated with various diseases such as emphysema, atherosclerosis, and inflammatory disorders.

Therefore, the Rat MMP12 ELISA Kit serves as a valuable tool for studying these conditions and potentially developing therapeutic interventions.With its reliable performance and ease of use, the Rat MMP12 ELISA Kit is a must-have for researchers investigating the role of MMP12 in disease pathology and exploring novel treatment strategies.

Product Name:Rat Matrix Metalloproteinase 12 (MMP12) ELISA Kit
Product Code:RTDL00679
Size:96 Assays
Target:MMP12
Synonyms:MME, HME, ME, Macrophage Metalloelastase, Macrophage Elastase
Detection Method:Sandwich
Range:78.125-5,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products