The Rat Matrix Metalloproteinase 11 (MMP11) ELISA Kit is designed for the accurate and precise measurement of MMP11 levels in rat samples including serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for your research needs.Matrix Metalloproteinase 11 (MMP11) is an enzyme that plays a crucial role in extracellular matrix remodeling and tissue repair processes. Studies have shown its involvement in various pathological conditions such as cancer metastasis, tissue fibrosis, and inflammatory diseases, highlighting its significance as a potential therapeutic target.
By using the Rat MMP11 ELISA Kit, researchers can gain valuable insights into the role of MMP11 in disease progression and response to treatments, ultimately aiding in the development of novel therapeutic strategies. With its ease of use and accurate quantification capabilities, this kit is an essential tool for advancing research in the field of matrix metalloproteinase biology.
Product Name:
Rat Matrix Metalloproteinase 11 (MMP11) ELISA Kit
Product Code:
RTDL00678
Size:
96 Assays
Target:
MMP11
Synonyms:
SL3, ST3, STMY3, Stromelysin 3
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
