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Rat Matrix Metalloproteinase 10 (MMP10) ELISA Kit

SKU:
RTDL00677
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P07152
Sensitivity:
0.053ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
STMY2,SL2,Stromelysin 2,Transin-2
Reactivity:
Rat
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Rat Matrix Metalloproteinase 10 (MMP10) ELISA Kit

The Rat Matrix Metalloproteinase-10 (MMP10) ELISA Kit is a reliable and accurate tool for the detection of MMP10 levels in rat samples. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.Matrix metalloproteinase-10 is an important enzyme involved in tissue remodeling and extracellular matrix degradation. It plays a crucial role in processes such as wound healing, inflammation, and cancer progression.

Monitoring MMP10 levels can provide valuable insights into the pathogenesis of various diseases and help in the development of therapeutic interventions.With easy-to-follow instructions and a user-friendly format, the Rat MMP10 ELISA Kit is suitable for both experienced researchers and beginners in the field. Trust this kit to deliver accurate and reliable results for your studies on MMP10 biology and its implications in disease progression.

Product Name:Rat Matrix Metalloproteinase 10 (MMP10) ELISA Kit
Product Code:RTDL00677
Size:96 Assays
Target:MMP10
Synonyms:STMY2, SL2, Stromelysin 2, Transin-2
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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