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Rat Matrix Metalloproteinase 1 (MMP1) ELISA Kit

SKU:
RTDL00676
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P81563
Sensitivity:
6.7pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CLGN,CLG1,Collagenase,Interstitial Collagenase,Vertebrate Collagenase,Fibroblast Collagenase
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Rat Matrix Metalloproteinase 1 (MMP1) ELISA Kit

The Rat Matrix Metalloproteinase-1 (MMP1) ELISA Kit is a powerful tool for the precise measurement of MMP1 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.MMP1 is a key enzyme in the breakdown of extracellular matrix proteins, playing a crucial role in tissue remodeling, wound healing, and inflammation. Dysregulation of MMP1 has been linked to a range of diseases, including cancer, arthritis, and cardiovascular disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.

With the Rat MMP1 ELISA Kit, researchers can confidently investigate the role of MMP1 in various physiological and pathological processes, ultimately advancing our understanding of disease mechanisms and identifying new treatment strategies.

Product Name:Rat Matrix Metalloproteinase 1 (MMP1) ELISA Kit
Product Code:RTDL00676
Size:96 Assays
Target:MMP1
Synonyms:CLGN, CLG1, Collagenase, Interstitial Collagenase, Vertebrate Collagenase, Fibroblast Collagenase
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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