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Rat Macrophage Stimulating Protein (MSP) ELISA Kit

SKU:
RTDL00693
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P70521
Sensitivity:
0.109ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
MST1,HGFL,NF15S2,Hepatocyte Growth Factor-Like Protein,Macrophage Stimulating 1
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat Macrophage Stimulating Protein (MSP) ELISA Kit

The Rat Macrophage Stimulating Protein (MSP) ELISA Kit is specifically designed for the accurate measurement of MSP levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.MSP is a key protein that plays a vital role in the immune response, particularly in the activation and regulation of macrophages. It is involved in various physiological and pathological processes, including inflammation, tissue repair, and immune function.

As such, MSP is an important biomarker for studying immune-related diseases, such as autoimmune disorders and infectious diseases, and for developing potential therapeutic interventions.Overall, the Rat MSP ELISA Kit provides researchers with a reliable tool for studying the role of MSP in immune responses and disease pathogenesis, allowing for valuable insights into potential treatment strategies.

Product Name:Rat Macrophage Stimulating Protein (MSP) ELISA Kit
Product Code:RTDL00693
Size:96 Assays
Target:MSP
Synonyms:MST1, HGFL, NF15S2, Hepatocyte Growth Factor-Like Protein, Macrophage Stimulating 1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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