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Rat Macrophage Inflammatory Protein 3 Beta (MIP3b) ELISA Kit

SKU:
RTDL00673
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
D3ZI84
Sensitivity:
6.6pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CCL19,CKb11,ELC,MIP3-B,SCYA19,Chemokine C-C-Motif Ligand 19,Beta Chemokine Exodus-3,CK Beta-11,EBI1-Ligand Chemokine,Small Inducible Cytokine Subfamily A19
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Rat Macrophage Inflammatory Protein 3 Beta (MIP3b) ELISA Kit

The Rat Macrophage Inflammatory Protein 3 Beta (MIP-3b) ELISA Kit is specifically designed for the quantitative detection of MIP-3b levels in rat serum, plasma, and tissue homogenates. This highly sensitive and specific kit ensures accurate and reproducible results, making it an essential tool for research in immunology, inflammation, and chemotaxis.MIP-3b, also known as CCL19, is a chemokine protein that plays a crucial role in recruiting immune cells to sites of inflammation and infection. It is involved in the regulation of immune responses, cell migration, and tissue homeostasis.

Elevated levels of MIP-3b have been associated with various inflammatory diseases, making it a valuable biomarker for studying immune responses and developing therapeutic interventions.Overall, the Rat MIP-3b ELISA Kit offers researchers a reliable and efficient way to analyze MIP-3b levels in rat samples, providing valuable insights into the role of this chemokine in inflammatory processes and immune system regulation.

Product Name:Rat Macrophage Inflammatory Protein 3 Beta (MIP3b) ELISA Kit
Product Code:RTDL00673
Size:96 Assays
Target:MIP3b
Synonyms:CCL19, CKb11, ELC, MIP3-B, SCYA19, Chemokine C-C-Motif Ligand 19, Beta Chemokine Exodus-3, CK Beta-11, EBI1-Ligand Chemokine, Small Inducible Cytokine Subfamily A19
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)91-9894
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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