Rat LRP-1 (Low-Density Lipoprotein-Receptor-Related Protein) CLIA Kit
The Rat LRP-1 (Low Density Lipoprotein Receptor Related Protein) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of LRP-1 levels in rat serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it ideal for various research applications.LRP-1 is a key protein involved in lipid metabolism and the regulation of cellular processes. It plays a crucial role in conditions such as cardiovascular diseases, Alzheimer's disease, and atherosclerosis, making it a valuable biomarker for studying these conditions and developing potential therapies.
Overall, the Rat LRP-1 CLIA Kit provides researchers with a powerful tool for studying LRP-1 biology and its implications in various disease states. Get accurate and reliable results with this innovative assay kit from Assay Genie.
Product Name:
Rat LRP-1 (Low-Density Lipoprotein-Receptor-Related Protein) CLIA Kit
SKU:
RTES00357
Target:
Rat LRP-1 (Low-Density Lipoprotein-Receptor-Related Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat LRP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LRP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LRP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat LRP-1. You can calculate the concentration of Rat LRP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
95-107
98-114
Average (%)
93
100
105
1:4
Range (%)
93-108
96-108
87-102
Average (%)
101
102
94
1:8
Range (%)
91-104
102-116
102-117
Average (%)
96
109
109
1:16
Range (%)
101-113
95-109
99-113
Average (%)
107
102
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
103
EDTA plasma (n=5)
91-103
97
Cell culture media (n=5)
99-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
435.16
1199.89
3220.12
450.86
1258.89
3488.72
Standard deviation
41.95
117.23
314.28
36.29
137.22
222.58
C V (%)
9.64
9.77
9.76
8.05
10.9
6.38
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat LRP-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat LRP-1 in samples. No significant cross-reactivity or interference between Rat LRP-1 and analogues was observed.
