Rat LpPLA2 (Lipoprotein-associated Phospholipase A2) ELISA Kit
The Rat LpPLA2 (Lipoprotein-associated Phospholipase A2) ELISA Kit is a specialized assay designed for the precise quantification of Lipoprotein-associated Phospholipase A2 levels in rat samples. Lipoprotein-associated Phospholipase A2 is an enzyme that plays a critical role in lipid metabolism, particularly in catalyzing the hydrolysis of phospholipids in low-density lipoproteins. This enzyme has been linked to various cardiovascular and inflammatory conditions, making it an essential biomarker for disease research and risk assessment.
Our ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for your experiments. Manufactured under strict quality control standards, this kit guarantees robust performance and ease of use, making it an excellent choice for studying the involvement of Lipoprotein-associated Phospholipase A2 in lipid metabolism and cardiovascular health. Trust Assay Genie's ELISA kits for reliable and high-quality measurement of key biomarkers in your research endeavors.
Product Name:
Rat LpPLA2 (Lipoprotein-associated Phospholipase A2) ELISA Kit
SKU:
AEES00469
Target:
Rat LpPLA2 (Lipoprotein-associated Phospholipase A2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 ng/mL
Detection range:
7.81-500 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat LpPLA2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LpPLA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LpPLA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat LpPLA2. You can calculate the concentration of Rat LpPLA2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
88-99
93-110
Average (%)
103
94
101
1:4
Range (%)
87-99
82-96
84-96
Average (%)
94
89
90
1:8
Range (%)
89-105
87-99
87-102
Average (%)
96
92
94
1:16
Range (%)
90-107
80-91
87-99
Average (%)
98
85
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
97
EDTA plasma (n=5)
89-103
95
Cell culture media (n=5)
87-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.61
73.68
223.37
26.64
72.46
243.51
Standard deviation
1.64
3.88
11.99
1.78
3.5
10.64
C V (%)
6.16
5.27
5.37
6.68
4.83
4.37
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat LpPLA2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat LpPLA2 in samples. No significant cross-reactivity or interference between Rat LpPLA2 and analogues was observed.
