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Rat Low Density Lipoprotein Receptor Related Protein 8 (LRP8) ELISA Kit

SKU:
RTDL00643
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
D3ZE75
Sensitivity:
13.3pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
APOER2,Apolipoprotein E Receptor 2
Reactivity:
Rat
Sample Type:
Serum, Plasma And Other Biological Fluids
€749
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Description

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Rat Low Density Lipoprotein Receptor Related Protein 8 (LRP8) ELISA Kit

The Rat Low Density Lipoprotein Receptor-Related Protein 8 (LRP8) ELISA Kit is a powerful tool for measuring levels of LRP8 in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.LRP8 is a key receptor involved in lipid metabolism and signal transduction, playing a critical role in the regulation of cholesterol homeostasis and neuronal development. Dysregulation of LRP8 has been implicated in a range of diseases, including Alzheimer's disease, atherosclerosis, and cancer, making it a valuable target for therapeutic interventions.

With its easy-to-use format and proven performance, the Rat LRP8 ELISA Kit is an essential tool for researchers seeking to better understand the physiological functions and pathological implications of LRP8 in rat models. Don't miss out on this valuable resource for your research endeavors.

Product Name:Rat Low Density Lipoprotein Receptor Related Protein 8 (LRP8) ELISA Kit
Product Code:RTDL00643
Size:96 Assays
Target:LRP8
Synonyms:APOER2, Apolipoprotein E Receptor 2
Detection Method:Sandwich
Range:31.25-2,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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