null

Rat Liver X Receptor Alpha (LXRa) ELISA Kit (RTDL00646)

SKU:
RTDL00646
Product Type:
ELISA Kit
Sensitivity:
0.112ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
NR1-H3, LXR-A,LXRA,RLD-1,Nuclear Receptor Subfamily 1,Group H,Member 3,Oxysterols receptor LXR-alpha
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749

Description

system_update_altDatasheet

Rat Liver X Receptor Alpha (LXRa) ELISA Kit

The Rat Liver X Receptor Alpha (LXRA) ELISA Kit is a powerful tool for the quantitative measurement of LXRA levels in rat samples, including serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and specificity, delivering accurate and consistent results for a variety of research purposes.LXRA is a key regulator of lipid metabolism and inflammation, playing a crucial role in various physiological and pathological processes.

Dysregulation of LXRA has been implicated in diseases such as atherosclerosis, obesity, and diabetes, highlighting its importance as a potential therapeutic target and biomarker for disease progression.With the Rat Liver X Receptor Alpha (LXRA) ELISA Kit, researchers can explore the role of LXRA in different biological pathways and diseases, paving the way for new insights and potential treatment options.

Product Name:Rat Liver X Receptor Alpha (LXRa) ELISA Kit
Product Code:RTDL00646
Size:96 Assays
Target:LXRa
Synonyms:NR1-H3, LXR-A, LXRA, RLD-1, Nuclear Receptor Subfamily 1,Group H,Member 3, Oxysterols receptor LXR-alpha
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products