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Rat Liver Receptor Homolog 1 (LRH1) ELISA Kit (RTDL00642)

SKU:
RTDL00642
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9QWM1
Sensitivity:
0.063ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
NR5A2,B1F,B1F2,CPF,FTF,FTZ-F1,LRH-1,hB1F,hB1F-2,Hepatocytic transcription factor,Alpha-1-fetoprotein transcription factor,Nuclear Receptor Subfamily 5,Group A,Member 2
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
€749

Description

system_update_altDatasheet

Rat Liver Receptor Homolog 1 (LRH1) ELISA Kit

The Rat Liver Receptor Homolog-1 (LRH1) ELISA Kit is a highly reliable and sensitive assay designed for the accurate measurement of LRH1 levels in rat liver tissue samples. This kit is ideal for researchers studying gene expression and transcriptional regulation in the liver, as LRH1 serves as a key transcription factor involved in lipid metabolism and bile acid synthesis.LRH1 plays a critical role in regulating various metabolic pathways, making it a valuable biomarker for understanding liver function and disease progression.

With its high specificity and reproducibility, researchers can trust the results obtained from this ELISA kit for their studies on liver physiology and metabolic disorders.Get your hands on the Rat Liver Receptor Homolog-1 (LRH1) ELISA Kit today to enhance your research capabilities and gain valuable insights into liver biology.

Product Name:Rat Liver Receptor Homolog 1 (LRH1) ELISA Kit
Product Code:RTDL00642
Size:96 Assays
Target:LRH1
Synonyms:NR5A2, B1F, B1F2, CPF, FTF, FTZ-F1, LRH-1, hB1F, hB1F-2, Hepatocytic transcription factor, Alpha-1-fetoprotein transcription factor, Nuclear Receptor Subfamily 5,Group A,Member 2
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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