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Rat Lipase, Pancreatic (PL) ELISA Kit

SKU:
RTDL00844
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P27657
Sensitivity:
37pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
PNLIP,PTL,Pancreatic Lipase,Pancreatic triacylglycerol lipase
Reactivity:
Rat
Sample Type:
Serum, Plasma And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Rat Lipase, Pancreatic (PL) ELISA Kit

The Rat Lipase Pancreatic PL ELISA Kit is specifically designed for the precise measurement of pancreatic lipase levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research studies.Pancreatic lipase is an important enzyme involved in the digestion and absorption of fats in the body. Its levels can vary in different conditions such as pancreatic diseases, obesity, and lipid metabolism disorders.

By measuring the levels of pancreatic lipase, researchers can gain valuable insights into these conditions and potential therapeutic interventions.Overall, the Rat Lipase Pancreatic PL ELISA Kit is a valuable tool for studying rat pancreatic lipase in both healthy and diseased states, providing researchers with the necessary information to advance their understanding of lipid metabolism and related disorders.

Product Name:Rat Lipase, Pancreatic (PL) ELISA Kit
Product Code:RTDL00844
Size:96 Assays
Target:PL
Synonyms:PNLIP, PTL, Pancreatic Lipase, Pancreatic triacylglycerol lipase
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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