Rat LIFR (Leukemia Inhibitory Factor Receptor) ELISA Kit
The Rat LIFR (Leukemia Inhibitory Factor Receptor) ELISA Kit is meticulously crafted for the precise measurement of LIFR levels in diverse rat biological samples.
LIFR is a multifaceted receptor involved in signaling pathways crucial for cellular processes like cell growth, differentiation, and survival. By quantifying LIFR expression, researchers can gain vital insights into its role in mediating leukemia inhibitory factor (LIF) signaling, immune regulation, and inflammatory responses. This ELISA kit empowers researchers to explore the intricate functions of LIFR and its impact on various physiological pathways. Accurate measurement of LIFR levels aids in deciphering the signaling mechanisms underlying cell proliferation, differentiation, and programmed cell death, offering valuable information for studying disease mechanisms and therapeutic targets. With exceptional sensitivity and specificity, the Rat LIFR ELISA Kit ensures reliable and reproducible results, allowing researchers to delve deeper into the molecular interactions involving LIFR. Manufactured under stringent quality control measures, this kit guarantees robust performance and user-friendly operation, making it an excellent choice for researchers investigating the complex roles of LIFR in cellular signaling pathways and disease processes.
Product Name:
Rat LIFR (Leukemia Inhibitory Factor Receptor) ELISA Kit
SKU:
AEES00557
Target:
Rat LIFR (Leukemia Inhibitory Factor Receptor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat LIFR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LIFR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LIFR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat LIFR. You can calculate the concentration of Rat LIFR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-108
91-103
90-104
Average (%)
98
96
97
1:4
Range (%)
89-104
88-103
94-106
Average (%)
95
95
100
1:8
Range (%)
87-102
87-100
92-102
Average (%)
94
93
97
1:16
Range (%)
88-104
92-103
88-98
Average (%)
95
97
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
91
EDTA plasma (n=5)
89-102
94
Cell culture media (n=5)
85-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.52
277.89
925.86
97.92
275.59
989.82
Standard deviation
5.73
13.31
43.24
5.36
15.68
51.37
C V (%)
5.64
4.79
4.67
5.47
5.69
5.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat LIFR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat LIFR in samples. No significant cross-reactivity or interference between Rat LIFR and analogues was observed.
