Rat LIF Recombinant Protein (RPPB0741)
- SKU:
- RPPB0741
- Product Type:
- Recombinant Protein
- Species:
- Rat
- Uniprot:
- P17777
- Research Area:
- Cytokines
Description
| Product Name: | Rat LIF Recombinant Protein |
| Product Code: | RPPB0741 |
| Size: | 10µg |
| Species: | Rat |
| Target: | LIF |
| Synonyms: | Leukemia inhibitory factor, Cholinergic neuronal differentiation factor, Lif. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | LIF Rat�was lyophilized from 0.2�m filtered concentrated solution in 1xPBS, pH 7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized Leukemia Inhibitory Factor (LIF) in sterile water not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Leukemia Inhibitory Factor (LIF) although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leukemia Inhibitory Factor (LIF) should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 96.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | SPLPITPVNA TCAIRHPCHG NLMNQIKSQL AQLNGSANAL FISYYTAQGE PFPNNVDKLC APNMTDFPPF HANGTEKTKL VELYRMVTYL GASLTNITWD QKNLNPTAVS LQIKLNATTD VMRGLLSSVL CRLCNKYHVG HVDVPCVPDN SSKEAFQRKK LGCQLLGTYK QVISVLAQAF |
| Biological Activity: | The activity of rat LIF is determined by the ability to induce differentiation of M1 myeloid leukemic cells. The minimum detectable concentration of rat LIF in this assay is 0.5ng/mL. |
Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence.
Leukemia Inhibitory Factor (LIF) Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19.8 kDa. The Leukemia Inhibitory Factor (LIF) is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Function: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. |
| NCBI Summary: | a secreted cytokine; involved in embryonic stem cell and myeloid cell growth and differentiation and stimulation of bone remodeling [RGD, Feb 2006] |
| UniProt Code: | P17777 |
| NCBI GenInfo Identifier: | 126281 |
| NCBI Gene ID: | 60584 |
| NCBI Accession: | P17777.1 |
| UniProt Related Accession: | P17777 |
| Molecular Weight: | 22,113 Da |
| NCBI Full Name: | Leukemia inhibitory factor |
| NCBI Synonym Full Names: | leukemia inhibitory factor |
| NCBI Official Symbol: | Lif�� |
| NCBI Protein Information: | leukemia inhibitory factor; cholinergic neuronal differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor) |
| UniProt Protein Name: | Leukemia inhibitory factor |
| UniProt Synonym Protein Names: | Cholinergic neuronal differentiation factor |
| Protein Family: | Leukemia inhibitory factor |
| UniProt Gene Name: | Lif�� |
| UniProt Entry Name: | LIF_RAT |
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