The Rat Luteinizing Hormone (LH) ELISA Kit is specifically designed for the quantitative measurement of LH levels in various rat biological samples. LH is a crucial hormone that plays a pivotal role in the regulation of reproductive processes, including ovulation and steroidogenesis. As a key gonadotropin, LH is essential for the maturation of ovarian follicles and the production of sex hormones, making it an important marker in reproductive endocrinology studies in rats. This ELISA kit from Assay Genie provides researchers with a reliable and sensitive tool for assessing LH levels, enabling detailed investigations into the reproductive hormone dynamics and fertility parameters in rat models.
The kit ensures high specificity and precision, allowing for accurate and reproducible results. Manufactured under strict quality control measures, this ELISA kit offers robust performance and user-friendly protocols, making it an excellent choice for researchers in the field of reproductive biology, endocrinology, and fertility studies.
Product Name:
Rat LH (Luteinizing Hormone) ELISA Kit
SKU:
AEES00301
Target:
Rat LH (Luteinizing Hormone)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 mIU/mL
Detection range:
1.56-100 mIU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat LH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat LH. You can calculate the concentration of Rat LH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
95-108
92-105
Average (%)
91
101
99
1:4
Range (%)
98-116
82-96
98-113
Average (%)
106
88
104
1:8
Range (%)
101-113
82-93
97-114
Average (%)
107
87
104
1:16
Range (%)
95-108
86-97
92-107
Average (%)
102
91
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
99
EDTA plasma (n=5)
93-105
98
Cell culture media (n=5)
90-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.7
15.4
40.8
5.0
15.1
38.8
Standard deviation
0.2
0.7
1.7
0.3
0.9
1.6
C V (%)
4.26
4.55
4.17
6.0
5.96
4.12
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat LH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat LH in samples. No significant cross-reactivity or interference between Rat LH and analogues was observed.
