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Rat Leucine Aminopeptidase (LAP) ELISA Kit

SKU:
RTDL00618
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q68FS4
Sensitivity:
0.145ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
LAPEP, LAP3,PEPS,Peptidase S,Leucyl Aminopeptidase,Cytosol aminopeptidase,Proline aminopeptidase,Prolyl aminopeptidase
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat Leucine Aminopeptidase (LAP) ELISA Kit

The Rat Leucine Aminopeptidase (LAP) ELISA Kit is a powerful tool for detecting and quantifying levels of LAP in rat samples. This kit offers unmatched sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.LAP is an important enzyme that plays a key role in protein degradation and amino acid metabolism. Its dysregulation has been implicated in various diseases and pathologies, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.

With the Rat LAP ELISA Kit, researchers can confidently measure LAP levels in serum, plasma, and cell culture supernatants, providing valuable insights into its role in physiological and pathological processes. Trust in the accuracy and precision of this kit for your research needs.

Product Name:Rat Leucine Aminopeptidase (LAP) ELISA Kit
Product Code:RTDL00618
Size:96 Assays
Target:LAP
Synonyms:LAPEP, LAP3, PEPS, Peptidase S, Leucyl Aminopeptidase, Cytosol aminopeptidase, Proline aminopeptidase, Prolyl aminopeptidase
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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