The Rat LDL (Low Density Lipoprotein) ELISA Kit is a specialized assay designed for the precise and quantitative measurement of low-density lipoprotein levels in various rat biological samples. Low-density lipoprotein, commonly known as LDL, is a crucial lipid transporter involved in the transport of cholesterol and its uptake by cells for essential physiological functions.
Abnormal levels of LDL are associated with various cardiovascular diseases and metabolic disorders, making its accurate measurement vital for understanding lipid metabolism and related conditions. This ELISA kit provides exceptional sensitivity and specificity, ensuring reliable and reproducible results in research studies focused on lipid metabolism, atherosclerosis, and cardiovascular health in rat models. Manufactured under stringent quality control standards by Assay Genie, this kit offers robust performance and is user-friendly, making it an excellent choice for scientific investigations into LDL-related pathways and diseases.
Product Name:
Rat LDL (Low Density Lipoprotein) ELISA Kit
SKU:
AEES00464
Target:
Rat LDL (Low Density Lipoprotein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 μg/mL
Detection range:
0.16-10 μg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat LDL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LDL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat LDL. You can calculate the concentration of Rat LDL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
94-109
98-112
Average (%)
94
101
103
1:4
Range (%)
101-114
88-102
93-108
Average (%)
107
94
101
1:8
Range (%)
98-114
81-94
94-105
Average (%)
106
87
99
1:16
Range (%)
98-110
81-92
90-104
Average (%)
103
87
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
94
EDTA plasma (n=5)
93-103
98
Cell culture media (n=5)
87-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.3
4.1
0.5
1.3
3.8
Standard deviation
0.03
0.07
0.22
0.03
0.06
0.11
C V (%)
6
5.38
5.37
6.0
4.62
2.89
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat LDL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat LDL in samples. No significant cross-reactivity or interference between Rat LDL and analogues was observed.
