Rat LBP (Lipopolysaccharide Binding Protein) ELISA Kit
The Rat LBP (Lipopolysaccharide Binding Protein) ELISA Kit is a powerful tool designed for the precise quantification of Lipopolysaccharide Binding Protein in rat samples. LBP is an essential component of the host defense mechanism, acting as a pattern recognition receptor involved in the immune response to bacterial infections. Its ability to bind and neutralize lipopolysaccharides (endotoxins) plays a pivotal role in innate immunity and inflammatory processes. Accurate measurement of LBP levels is crucial for understanding its function in host defense, infection, and inflammation.
Our ELISA kit ensures exceptional sensitivity and specificity, enabling dependable and reproducible results for your research requirements. Manufactured with stringent quality control measures, this kit delivers robust performance and user-friendly operation, making it an ideal choice for investigating the role of LBP in immune responses and inflammatory pathways. Trust Assay Genie's ELISA kits for high-quality and accurate measurement of key biomarkers in your research studies.
Product Name:
Rat LBP (Lipopolysaccharide Binding Protein) ELISA Kit
SKU:
AEES00468
Target:
Rat LBP (Lipopolysaccharide Binding Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.13 ng/mL
Detection range:
1.88-120 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat LBP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LBP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat LBP. You can calculate the concentration of Rat LBP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
85-97
94-109
Average (%)
99
90
101
1:4
Range (%)
91-105
79-93
86-96
Average (%)
97
85
91
1:8
Range (%)
93-106
87-97
84-95
Average (%)
100
92
90
1:16
Range (%)
87-99
85-99
82-97
Average (%)
94
91
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
94
EDTA plasma (n=5)
91-106
99
Cell culture media (n=5)
91-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
6.15
17.16
56.61
6.59
18.22
58.9
Standard deviation
0.32
0.98
2.94
0.39
1.04
2.93
C V (%)
5.2
5.71
5.19
5.92
5.71
4.97
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat LBP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat LBP in samples. No significant cross-reactivity or interference between Rat LBP and analogues was observed.
