The Rat Kruppel-Like Factor 2 (KLF2) Lung ELISA kit is specifically designed for the precise measurement of KLF2 levels in rat lung tissue homogenates. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it a valuable tool for various research investigations.KLF2, a transcription factor, plays a vital role in regulating gene expression in endothelial cells and is involved in various physiological processes such as vascular development and inflammation. Dysregulation of KLF2 has been linked to several diseases, including pulmonary hypertension and lung injury, making it a key target for studying these conditions and developing potential therapeutic interventions.
Whether you're studying lung-related disorders, vascular biology, or transcriptional regulation, the Rat KLF2 Lung ELISA Kit provides a reliable and efficient method for quantifying KLF2 levels in rat lung samples, facilitating your research efforts and aiding in the discovery of novel insights into disease mechanisms.
Product Name:
Rat Kruppel Like Factor 2, Lung (KLF2) ELISA Kit
Product Code:
RTDL00608
Size:
96 Assays
Target:
KLF2
Synonyms:
LKLF, Lung krueppel-like factor
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
