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Rat Kisspeptin Receptor (KISS1R) ELISA Kit

SKU:
RTDL00606
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q924U1
Sensitivity:
0.064ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
AXOR12,GPR54,KiSS1-Derived Peptide Receptor,G Protein-Coupled Receptor 54,Hypogonadotropin-1,Kisspeptins receptor,Metastin receptor
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Rat Kisspeptin Receptor (KISS1R) ELISA Kit

The Rat Kisspeptin Receptor (Kiss1R) ELISA Kit is a powerful tool for the accurate measurement of Kiss1R levels in rat samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides dependable and consistent results, making it perfect for a variety of research purposes.Kisspeptin receptor, also known as Kiss1R, is a key player in the regulation of reproductive function, including the control of puberty and fertility.

Dysregulation of this receptor has been implicated in various reproductive disorders, making it a valuable biomarker for studying these conditions and developing potential treatments.Don't miss out on the opportunity to advance your research with the Rat Kisspeptin Receptor (Kiss1R) ELISA Kit from AssayGenie.

Product Name:Rat Kisspeptin Receptor (KISS1R) ELISA Kit
Product Code:RTDL00606
Size:96 Assays
Target:KISS1R
Synonyms:AXOR12, GPR54, KiSS1-Derived Peptide Receptor, G Protein-Coupled Receptor 54, Hypogonadotropin-1, Kisspeptins receptor, Metastin receptor
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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