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Rat Kallikrein 4 (KLK4) ELISA Kit

SKU:
RTDL00611
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q6IE12
Sensitivity:
0.052ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
ARM1,EMSP1,KLK-L1,PRSS17,PSTS,Enamel Matrix,Prostate,Kallikrein-Related Peptidase 4,Enamel matrix serine proteinase 1,Serine protease 17,Kallikrein-like protein 1
Reactivity:
Rat
Sample Type:
Serum, Plasma And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Rat Kallikrein 4 (KLK4) ELISA Kit

The Rat KLK4 (Kallikrein 4) ELISA Kit is a high-quality assay designed for the precise quantification of KLK4 levels in rat serum, plasma, and tissue homogenates. This kit offers outstanding sensitivity and specificity, ensuring robust and consistent results for various research applications.KLK4 is a serine protease that plays a critical role in a variety of physiological and pathological processes, including proteolysis, ECM remodeling, and cancer progression.

Elevated levels of KLK4 have been associated with various cancers, making it a valuable biomarker for understanding disease mechanisms and developing targeted therapies.Overall, the Rat KLK4 ELISA Kit provides researchers with a reliable tool to study KLK4 expression levels in rat models, offering valuable insights into its roles in health and disease.

Product Name:Rat Kallikrein 4 (KLK4) ELISA Kit
Product Code:RTDL00611
Size:96 Assays
Target:KLK4
Synonyms:ARM1, EMSP1, KLK-L1, PRSS17, PSTS, Enamel Matrix,Prostate, Kallikrein-Related Peptidase 4, Enamel matrix serine proteinase 1, Serine protease 17, Kallikrein-like protein 1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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