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Rat Kallikrein 3 (KLK3) ELISA Kit

SKU:
RTDL00610
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P15950
Sensitivity:
0.057ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
APS,KLK2A1,PSA,HK3,KLKB1,Seminin,Prostate Specific Antigen,Kallikrein-Related Peptidase 3,Semenogelase, g-Seminoprotein,P-30 Antigen,Plasma kallikrein
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

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Rat Kallikrein 3 (KLK3) ELISA Kit

The Rat KLK3 (Kallikrein 3) ELISA Kit is specifically designed to accurately measure KLK3 levels in rat serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, providing researchers with reliable and consistent results for various research applications.KLK3, also known as prostate-specific antigen (PSA), is an important biomarker for prostate cancer detection and monitoring. Elevated levels of KLK3 in the blood can indicate the presence of prostate cancer or other prostate-related conditions.

By using this ELISA kit, researchers can study KLK3 levels in rat models to better understand its role in disease progression and develop potential therapeutic interventions.Overall, the Rat KLK3 ELISA Kit is an essential tool for researchers studying prostate cancer and other prostate-related diseases in rat models, offering precise and accurate measurements of KLK3 levels for impactful research outcomes.

Product Name:Rat Kallikrein 3 (KLK3) ELISA Kit
Product Code:RTDL00610
Size:96 Assays
Target:KLK3
Synonyms:APS, KLK2A1, PSA, HK3, KLKB1, Seminin, Prostate Specific Antigen, Kallikrein-Related Peptidase 3, Semenogelase, g-Seminoprotein, P-30 Antigen, Plasma kallikrein
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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