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Rat Kallikrein 1 (KLK1) ELISA Kit

SKU:
RTDL00609
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P00758
Sensitivity:
0.064ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
KLKR,Klk6,HK1,Kallikrein 1,Renal/Pancreas/Salivary,Kidney/pancreas/salivary gland kallikrein,Tissue kallikrein
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Rat Kallikrein 1 (KLK1) ELISA Kit

The Rat Kallikrein-1 (KLK1) ELISA Kit is a powerful tool for detecting and quantifying KLK1 levels in rat serum, plasma, and tissue homogenates. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it ideal for a wide range of research applications. KLK1 is a key enzyme involved in various physiological processes, including blood pressure regulation, inflammation, and tissue remodeling.

Dysregulation of KLK1 has been implicated in conditions such as hypertension, kidney disease, and inflammatory disorders, highlighting its importance as a potential biomarker for studying and treating these diseases. By using the Rat KLK1 ELISA Kit, researchers can gain valuable insights into the role of KLK1 in health and disease, ultimately paving the way for the development of new therapeutic strategies targeting this enzyme.

Product Name:Rat Kallikrein 1 (KLK1) ELISA Kit
Product Code:RTDL00609
Size:96 Assays
Target:KLK1
Synonyms:KLKR, Klk6, HK1, Kallikrein 1,Renal/Pancreas/Salivary, Kidney/pancreas/salivary gland kallikrein, Tissue kallikrein
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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