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Rat Isocitrate Dehydrogenase 1, Soluble (IDH1) ELISA Kit

SKU:
RTDL00521
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P41562
Sensitivity:
0.116ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
PICD,IDP,Oxalosuccinate decarboxylase,NADP(+)-specific ICDH,Cytosolic NADP-isocitrate dehydrogenase
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
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Description

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Rat Isocitrate Dehydrogenase 1, Soluble (IDH1) ELISA Kit

The Rat Isocitrate Dehydrogenase 1 (Soluble IDH1) ELISA Kit is a reliable and accurate assay designed for the precise detection of soluble IDH1 levels in rat serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring dependable and reproducible results for a variety of research applications.Soluble IDH1 is an enzyme that plays a crucial role in cellular metabolism and has been linked to various diseases, including cancer and neurodegenerative disorders.

By measuring soluble IDH1 levels, researchers can gain valuable insights into disease mechanisms and potentially identify new therapeutic targets.Overall, the Rat Isocitrate Dehydrogenase 1 (Soluble IDH1) ELISA Kit is a valuable tool for studying the role of soluble IDH1 in disease pathology and developing novel treatment strategies.

Product Name:Rat Isocitrate Dehydrogenase 1, Soluble (IDH1) ELISA Kit
Product Code:RTDL00521
Size:96 Assays
Target:IDH1
Synonyms:PICD, IDP, Oxalosuccinate decarboxylase, NADP(+)-specific ICDH, Cytosolic NADP-isocitrate dehydrogenase
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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