The Rat Irisin ELISA Kit is specifically developed for the precise quantitative measurement of irisin levels in various rat biological samples. Irisin is a novel myokine that has gained significant interest due to its potential role in regulating energy metabolism, thermogenesis, and exercise-related physiology. This ELISA kit enables researchers to accurately quantify irisin concentrations, aiding investigations into the physiological effects of this hormone on metabolism and overall health. Accurate measurement of irisin levels using the Rat Irisin ELISA Kit is essential for understanding its involvement in metabolic processes, obesity, exercise physiology, and other related research areas.
Researchers can utilize this kit to explore the impact of irisin on energy homeostasis, muscle function, and metabolic disorders, providing valuable insights into potential therapeutic strategies targeting metabolic diseases and related conditions. Assay Genie's Rat Irisin ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit delivers robust performance and user-friendly operation, making it an excellent choice for researchers investigating the physiological functions of irisin and its implications in metabolic health.
Product Name:
Rat Irisin ELISA Kit
SKU:
AEES00514
Target:
Rat Irisin
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Irisin. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Irisin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Irisin, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat Irisin. You can calculate the concentration of Rat Irisin in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-112
85-97
94-111
Average (%)
103
90
101
1:4
Range (%)
94-111
82-98
90-105
Average (%)
102
89
96
1:8
Range (%)
99-113
85-100
97-111
Average (%)
105
91
104
1:16
Range (%)
93-110
84-98
91-106
Average (%)
101
90
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-105
96
EDTA plasma (n=5)
86-99
92
Cell culture media (n=5)
89-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
263.28
561.8
1801.01
261.18
528.07
1634.15
Standard deviation
17.61
28.88
92.39
13.89
30.94
78.44
C V (%)
6.69
5.14
5.13
5.32
5.86
4.8
Sample type &Sample volume:
; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat Irisin concentrations in .
Specificity:
This kit recognizes Rat Irisin in samples. No significant cross-reactivity or interference between Rat Irisin and analogues was observed.
