The Rat Interleukin-9 (IL9) ELISA Kit is specifically designed for the accurate quantification of interleukin-9 levels in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it an ideal tool for a variety of research applications.Interleukin-9 is a key cytokine involved in immune responses, particularly in the regulation of allergic reactions and inflammation. Its dysregulation has been linked to various diseases, including asthma, autoimmune disorders, and inflammatory conditions.
Therefore, measuring IL9 levels can provide valuable insights into the pathogenesis of these diseases and facilitate the development of targeted therapies.The Rat Interleukin-9 (IL9) ELISA Kit from Assay Genie is a reliable and versatile tool for researchers looking to study the role of IL9 in rat models, offering a convenient and effective solution for measuring this important cytokine in biological samples.
Product Name:
Rat Interleukin 9 (IL-9) ELISA Kit
Product Code:
RTDL00574
Size:
96 Assays
Target:
IL9
Synonyms:
HP40, P40, TCGFIII, TCGF3, T Cell Growth Factor III, P40 T-Cell And Mast Cell Growth Factor, P40 Cytokine, Homolog Of Mouse T Cell And Mast Cell Growth Factor 40
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
