null

Rat Interleukin 6 Receptor (IL-6R) ELISA Kit

SKU:
RTDL00569
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P22273
Sensitivity:
0.25ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
CD126,gp80,IL6R,IL-6R-1,IL-6R-Alpha,IL6RA,IL-6 receptor subunit alpha,Membrane glycoprotein 80
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat Interleukin 6 Receptor (IL-6R) ELISA Kit

The Rat Interleukin-6 Receptor (IL6R) ELISA Kit is a reliable tool for measuring levels of IL6R in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it suitable for a variety of research applications.IL6R is a key receptor involved in the immune response and inflammation processes. It plays a crucial role in various diseases such as autoimmune disorders, cancer, and inflammatory conditions.

Studying IL6R levels can provide valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Rat Interleukin-6 Receptor (IL6R) ELISA Kit is an essential tool for researchers looking to investigate the role of IL6R in health and disease in rat models.

Product Name:Rat Interleukin 6 Receptor (IL-6R) ELISA Kit
Product Code:RTDL00569
Size:96 Assays
Target:IL6R
Synonyms:CD126, gp80, IL6R, IL-6R-1, IL-6R-Alpha, IL6RA, IL-6 receptor subunit alpha, Membrane glycoprotein 80
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-9994
Heparin plasma (n=5)91-10196
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products