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Rat Interleukin 2 Receptor Alpha (IL-2Ra) ELISA Kit

SKU:
RTDL00562
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P26897
Sensitivity:
0.051ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD25,IL2R-a,IL2-RA,IDDM10,IL2R,TCGFR,p55,TAC antigen
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat Interleukin 2 Receptor Alpha (IL-2Ra) ELISA Kit

The Rat Interleukin-2 Receptor Alpha (IL2RA) ELISA Kit is a powerful tool for detecting and quantifying IL2RA levels in rat samples, including serum, plasma, and cell culture supernatants. This highly sensitive and specific kit provides accurate and reliable results for a variety of research applications.IL2RA is a key receptor involved in the immune response, specifically in regulating T cell activation and proliferation. Dysregulation of IL2RA has been implicated in various immune disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.

With its user-friendly protocol and robust performance, the Rat IL2RA ELISA Kit is an essential asset for researchers investigating the role of IL2RA in immunology and related fields. Trust Assay Genie for quality ELISA kits that deliver consistent and precise results for your research needs.

Product Name:Rat Interleukin 2 Receptor Alpha (IL-2Ra) ELISA Kit
Product Code:RTDL00562
Size:96 Assays
Target:IL2Ra
Synonyms:CD25, IL2R-a, IL2-RA, IDDM10, IL2R, TCGFR, p55, TAC antigen
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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