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Rat Interleukin 12A (IL-12A) ELISA Kit

SKU:
RTDL00541
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9R278
Sensitivity:
6.1pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
p35,IL12-A,CLMF1,NFSK,NKSF1,Natural Killer Cell Stimulatory Factor 1,Cytotoxic Lymphocyte Maturation Factor 1,NF Cell Stimulatory Factor Chain 1
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat Interleukin 12A (IL-12A) ELISA Kit

The Rat Interleukin-12A (IL-12A) ELISA Kit is specifically designed for the accurate detection of interleukin-12A levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.Interleukin-12A is an important cytokine involved in the immune response, regulating the differentiation of T cells and promoting the production of interferon-gamma. Dysregulation of interleukin-12A has been implicated in various inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and developing potential therapies.

With its advanced technology and optimized protocols, the Rat Interleukin-12A (IL-12A) ELISA Kit provides researchers with a powerful tool for investigating the role of interleukin-12A in immune responses and disease pathology. Trust AssayGenie for accurate and reliable results in your research endeavors.

Product Name:Rat Interleukin 12A (IL-12A) ELISA Kit
Product Code:RTDL00541
Size:96 Assays
Target:IL12A
Synonyms:p35, IL12-A, CLMF1, NFSK, NKSF1, Natural Killer Cell Stimulatory Factor 1, Cytotoxic Lymphocyte Maturation Factor 1, NF Cell Stimulatory Factor Chain 1
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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