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Rat Interferon Regulatory Factor 4 (IRF4) ELISA Kit

SKU:
RTDL00586
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
D3ZEX6
Sensitivity:
0.133ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
LSIRF,MUM1,NF-EM5,Lymphocyte-specific interferon regulatory factor,Multiple myeloma oncogene 1
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat Interferon Regulatory Factor 4 (IRF4) ELISA Kit

The Rat Interferon Regulatory Factor 4 (IRF4) ELISA Kit is specifically designed for the precise measurement of IRF4 levels in rat samples, including serum, plasma, and tissue homogenates. This kit provides exceptional sensitivity and specificity, ensuring accurate and reproducible results that are perfect for a variety of research applications.IRF4 is a critical transcription factor involved in the regulation of immune responses and is essential for the development and function of immune cells.

Dysregulation of IRF4 has been linked to various autoimmune diseases, inflammatory disorders, and cancers, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.By using the Rat IRF4 ELISA Kit, researchers can gain valuable insights into the role of IRF4 in immune system function and disease pathogenesis, facilitating innovative research and the development of novel treatment strategies.

Product Name:Rat Interferon Regulatory Factor 4 (IRF4) ELISA Kit
Product Code:RTDL00586
Size:96 Assays
Synonyms:LSIRF, MUM1, NF-EM5, Lymphocyte-specific interferon regulatory factor, Multiple myeloma oncogene 1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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