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Rat Integrin Alpha M (ITGaM) ELISA Kit

SKU:
RTDL00593
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
G3V8L7
Sensitivity:
0.115ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CD11b,ITG-AM,CR3A,MAC-1,MAC1A,MO1A,Complement Component 3 Receptor 3 Subunit,Cell surface glycoprotein MAC-1 subunit alpha,Neutrophil adherence receptor
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

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Rat Integrin Alpha M (ITGaM) ELISA Kit

The Rat Integrin Alpha M (ITGAM) ELISA Kit is specifically designed for the precise measurement of ITGAM levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and accuracy, guaranteeing dependable and consistent results for various research purposes.Integrin Alpha M (ITGAM) is a key protein involved in cell adhesion and migration, playing a crucial role in inflammatory responses and immune cell function. Dysregulation of ITGAM has been implicated in various diseases, including autoimmune disorders and certain types of cancer.

Therefore, measuring ITGAM levels using this ELISA kit can provide valuable insights into disease mechanisms and potential therapeutic interventions.Overall, the Rat Integrin Alpha M (ITGAM) ELISA Kit is a valuable tool for researchers studying immune response, inflammation, and disease pathology in rat models, offering reliable and accurate measurements of ITGAM levels.

Product Name:Rat Integrin Alpha M (ITGaM) ELISA Kit
Product Code:RTDL00593
Size:96 Assays
Target:ITGaM
Synonyms:CD11b, ITG-AM, CR3A, MAC-1, MAC1A, MO1A, Complement Component 3 Receptor 3 Subunit, Cell surface glycoprotein MAC-1 subunit alpha, Neutrophil adherence receptor
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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