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Rat Insulin Like Growth Factor 1 (IGF1) ELISA Kit

SKU:
RTDL00526
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P08025
Sensitivity:
30pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
IGFI,IGF1A,IBP1,MGF,Somatomedin C,Mechano Growth Gactor
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Rat Insulin Like Growth Factor 1 (IGF1) ELISA Kit

The Rat Insulin-like Growth Factor 1 (IGF1) ELISA Kit is a reliable and accurate tool for measuring IGF1 levels in rat serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.IGF1 is a key hormone that plays a crucial role in growth, development, and metabolism. It influences cell growth and differentiation, and is involved in regulating various physiological processes.

Abnormal levels of IGF1 have been linked to conditions such as diabetes, Alzheimer's disease, and cancer, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.With its ease of use and robust performance, the Rat IGF1 ELISA Kit is an essential tool for researchers investigating the role of IGF1 in various biological processes and disease models.

Product Name:Rat Insulin Like Growth Factor 1 (IGF1) ELISA Kit
Product Code:RTDL00526
Size:96 Assays
Target:IGF1
Synonyms:IGFI, IGF1A, IBP1, MGF, Somatomedin C, Mechano Growth Gactor
Detection Method:Sandwich
Range:78.125-5,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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