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Rat Insulin Degrading Enzyme (IDE) ELISA Kit

SKU:
RTDL00520
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P35559
Sensitivity:
0.129ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
Insulysin,Insulin Protease,Abeta-degrading protease,Insulinase
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Rat Insulin Degrading Enzyme (IDE) ELISA Kit

The Rat Insulin Degrading Enzyme (IDE) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of IDE levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.IDE is an important enzyme that plays a key role in the degradation of insulin and amyloid beta peptides, making it a crucial player in insulin metabolism and the pathogenesis of diseases such as diabetes and Alzheimer's disease.

Studying IDE levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.With its high sensitivity and specificity, the Rat IDE ELISA Kit is a valuable tool for researchers studying the mechanisms underlying insulin metabolism, neurodegenerative disorders, and other related conditions in rat models. Order yours today to advance your research efforts.

Product Name:Rat Insulin Degrading Enzyme (IDE) ELISA Kit
Product Code:RTDL00520
Size:96 Assays
Target:IDE
Synonyms:Insulysin, Insulin Protease, Abeta-degrading protease, Insulinase
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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