The Rat INS (Insulin) ELISA Kit is a precision-designed assay developed for the quantitative detection and measurement of insulin levels in rat samples. Insulin is a critical hormone involved in regulating blood glucose levels, carbohydrate metabolism, and energy balance. This ELISA kit provides researchers with a reliable method to quantify insulin concentrations accurately, facilitating studies related to diabetes, metabolism, and endocrine function. Accurate measurement of insulin levels using this ELISA kit is vital for investigating various physiological and pathological processes, including glucose homeostasis, insulin resistance, and diabetes mellitus.
By enabling precise quantification of insulin, researchers can gain insights into the mechanisms underlying metabolic disorders and explore potential therapeutic strategies aimed at managing diabetes and related conditions. The Rat INS ELISA Kit offered by Assay Genie ensures exceptional sensitivity and specificity, guaranteeing dependable and reproducible results. Manufactured under strict quality control standards, this kit delivers robust performance and user-friendly operation, making it an ideal choice for researchers conducting studies on insulin and its role in metabolic regulation and disease states.
Product Name:
Rat INS (Insulin) ELISA Kit
SKU:
AEES00513
Target:
Rat INS (Insulin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat INS. You can calculate the concentration of Rat INS in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
-
-
96-112
Average (%)
-
-
102
1:4
Range (%)
-
-
90-101
Average (%)
-
-
95
1:8
Range (%)
-
-
84-96
Average (%)
-
-
89
1:16
Range (%)
-
-
86-98
Average (%)
-
-
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
-
-
EDTA plasma (n=5)
-
-
Cell culture media (n=5)
86-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.99
2.68
6.73
1.0
2.82
6.9
Standard deviation
0.06
0.14
0.25
0.07
0.16
0.35
C V (%)
6.06
5.22
3.71
7.0
5.67
5.07
Sample type &Sample volume:
cell culture supernatant and tissue homogenate; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat INS concentrations in cell culture supernatant and tissue homogenate.
Specificity:
This kit recognizes Rat INS in samples. No significant cross-reactivity or interference between Rat INS and analogues was observed.
