IGSF1,IGCD1,IGDC1,INHBP,PGSF2,p120,Immunoglobulin Superfamily Member 1,Immunoglobulin-like domain-containing protein 1,Pituitary gland-specific factor 2
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
The Rat Inhibin Binding Protein (INHBP) ELISA Kit is specifically designed for the accurate measurement of INHBP levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.INHBP is a vital protein that binds to inhibin, a hormone that regulates the production of follicle-stimulating hormone in the pituitary gland. By measuring INHBP levels, researchers can gain insights into the regulation of reproductive function and hormone signaling pathways in rats.
This information is valuable for studying reproductive disorders, hormone-related diseases, and potential therapeutic interventions.Overall, the Rat INHBP ELISA Kit provides a reliable tool for researchers to investigate the role of INHBP in rat physiology and pathology, offering valuable data for advancing our understanding of hormonal regulation and reproductive health in animal models.
Product Name:
Rat Inhibin Binding Protein (INHBP) ELISA Kit
Product Code:
RTDL00579
Size:
96 Assays
Target:
INHBP
Synonyms:
IGSF1, IGCD1, IGDC1, INHBP, PGSF2, p120, Immunoglobulin Superfamily Member 1, Immunoglobulin-like domain-containing protein 1, Pituitary gland-specific factor 2
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
