The Rat IL-17A ELISA Kit is meticulously designed to facilitate the precise and quantitative determination of Interleukin-17A (IL-17A) levels in diverse rat biological samples. IL-17A serves as a pivotal pro-inflammatory cytokine, playing a crucial role in orchestrating immune responses, inflammation, and defense mechanisms.
Its involvement spans a spectrum of immune-related functions, contributing significantly to the regulation of inflammatory cascades and immune cell activation. Designed by Assay Genie, the IL-17A ELISA Kit ensures unparalleled sensitivity and specificity, guaranteeing the generation of accurate and reproducible results. With a commitment to stringent quality control measures, this kit delivers robust performance and user-friendly operation, rendering it an indispensable tool for researchers aiming to unravel the complex roles of IL-17A in various physiological and pathological processes.
Product Name:
Rat IL-17A ELISA Kit
SKU:
AEES00457
Target:
Rat IL-17A
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-17A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-17A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-17A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-17A. You can calculate the concentration of Rat IL-17A in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
87-100
98-113
Average (%)
94
94
105
1:4
Range (%)
99-114
85-95
96-112
Average (%)
106
90
102
1:8
Range (%)
99-115
83-95
98-113
Average (%)
107
90
104
1:16
Range (%)
100-113
85-98
92-108
Average (%)
106
91
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-110
101
EDTA plasma (n=5)
97-111
102
Cell culture media (n=5)
93-104
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
55.2
149.7
486.2
50.5
153.3
468.5
Standard deviation
3.4
7.9
18.0
2.6
8.7
21.6
C V (%)
6.16
5.28
3.7
5.15
5.68
4.61
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IL-17A concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IL-17A in samples. No significant cross-reactivity or interference between Rat IL-17A and analogues was observed.
