Rat IL 10 Recombinant Protein (RPPB0556)
- SKU:
- RPPB0556
- Product Type:
- Recombinant Protein
- Species:
- Rat
- Uniprot:
- P29456
- Research Area:
- Cytokines
Description
| Product Name: | Rat IL 10 Recombinant Protein |
| Product Code: | RPPB0556 |
| Size: | 10µg |
| Species: | Rat |
| Target: | IL 10 |
| Synonyms: | B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The protein was lyophilized containing 20mM Tris-HCl pH-8.0 and 100mM NaCl. |
| Solubility: | It is recommended to reconstitute the lyophilized IL-10 Rat Recombinant in sterile 18M?-cm H2O not less than 100 �g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized IL-10 Rat although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Interleukin10 Rat recombinant should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | SKGHSIRGDN NCTHFPVSQT HMLRELRAAF SQVKTFFQKK DQLDNILLTD SLLQDFKGYL GCQALSEMIK FYLVEVMPQA ENHGPEIKEH LNSLGEKLKT LWIQLRRCHR FLPCENKSKA VEQVKNDFNK LQDKGVYKAM NEFDIFINCI EAYVTLKMKN |
| Biological Activity: | The ED50 as determined by the dose dependent inhibition of MC/9 proliferation is less than 10ng/ml concentration corresponding to a Specific Activity of 100,000IU/mg. |
IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
IL-10 Recombinant Rat produced in E.coli is a single, glycosylated polypeptide chain containing 160 amino acids and having a molecular mass of 18.6 kDa. The Interleukin-10 Mouse is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. |
| NCBI Summary: | factor involved in the inhibition of cytokine synthesis [RGD, Feb 2006] |
| UniProt Code: | P29456 |
| NCBI GenInfo Identifier: | 266338 |
| NCBI Gene ID: | 25325 |
| NCBI Accession: | P29456.1 |
| UniProt Secondary Accession: | P29456,Q63263, |
| UniProt Related Accession: | P29456 |
| Molecular Weight: | 20,426 Da |
| NCBI Full Name: | Interleukin-10 |
| NCBI Synonym Full Names: | interleukin 10 |
| NCBI Official Symbol: | Il10�� |
| NCBI Official Synonym Symbols: | IL10X�� |
| NCBI Protein Information: | interleukin-10; CSIF; IL-10; cytokine synthesis inhibitory factor |
| UniProt Protein Name: | Interleukin-10 |
| UniProt Synonym Protein Names: | Cytokine synthesis inhibitory factor; CSIF |
| Protein Family: | Interleukin |
| UniProt Gene Name: | Il10�� |
| UniProt Entry Name: | IL10_RAT |
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