The Rat IgM (Immunoglobulin M) ELISA Kit is a state-of-the-art assay designed to quantitatively measure the levels of IgM, a critical component of the immune system, in rat biological samples.
IgM is the first antibody produced during the initial immune response to infection, playing a pivotal role in neutralizing pathogens and activating the immune system. Accurate measurement of IgM levels is essential for understanding immune function and immune responses in rats, providing valuable insights into infection, autoimmunity, and inflammatory conditions. This advanced ELISA kit enables researchers to precisely quantify IgM levels in rat samples, shedding light on the immune status and responses in experimental models. IgM has significant implications in immune defense mechanisms and disease pathogenesis, making the Rat IgM ELISA Kit a valuable tool for immunology research and preclinical studies. With exceptional sensitivity and specificity, the Rat IgM ELISA Kit from Assay Genie ensures precise and reproducible results. Manufactured under strict quality control standards, this kit delivers reliable performance and ease of use, making it an indispensable choice for researchers investigating the role of IgM in immune function and disease processes in rat models.
Product Name:
Rat IgM (Immunoglobulin M) ELISA Kit
SKU:
AEES00660
Target:
Rat IgM (Immunoglobulin M)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IgM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IgM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IgM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IgM. You can calculate the concentration of Rat IgM in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
97-111
86-96
Average (%)
94
105
91
1:4
Range (%)
86-98
94-109
87-97
Average (%)
91
100
92
1:8
Range (%)
86-102
96-109
83-96
Average (%)
93
102
88
1:16
Range (%)
89-104
97-113
85-98
Average (%)
96
103
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
90
EDTA plasma (n=5)
95-108
102
Cell culture media (n=5)
90-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.25
4.79
0.46
1.34
4.53
Standard deviation
0.03
0.07
0.23
0.03
0.06
0.25
C V (%)
5.92
5.63
4.78
6.92
4.19
5.49
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IgM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IgM in samples. No significant cross-reactivity or interference between Rat IgM and analogues was observed.
