Rat IGFBP-1 (Insulin-Like Growth Factor Binding Protein 1) CLIA Kit
The Rat IGFBP-1 (Insulin-like Growth Factor Binding Protein-1) CLIA Kit is specifically designed for the quantitative detection of IGFBP-1 levels in rat samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.IGFBP-1 is a key regulator of insulin-like growth factor activity, playing a crucial role in cell growth, differentiation, and metabolism. Dysregulation of IGFBP-1 has been associated with various diseases such as diabetes, cardiovascular disorders, and cancer, highlighting its importance as a potential biomarker for disease diagnosis and treatment development.
With the Rat IGFBP-1 CLIA Kit, researchers can effectively study the role of IGFBP-1 in disease pathogenesis and progression, leading to a better understanding of its therapeutic implications. This kit provides a valuable tool for researchers in the field of molecular biology and biomedical science.
Product Name:
Rat IGFBP-1 (Insulin-Like Growth Factor Binding Protein 1) CLIA Kit
SKU:
RTES00316
Target:
Rat IGFBP-1 (Insulin-Like Growth Factor Binding Protein 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IGFBP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IGFBP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IGFBP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IGFBP-1. You can calculate the concentration of Rat IGFBP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-110
88-100
98-114
Average (%)
104
93
104
1:4
Range (%)
100-116
99-113
91-102
Average (%)
106
105
97
1:8
Range (%)
89-100
99-114
93-108
Average (%)
94
107
101
1:16
Range (%)
100-115
88-103
84-97
Average (%)
106
95
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-104
96
EDTA plasma (n=5)
97-111
103
Cell culture media (n=5)
101-117
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.22
87.27
376.98
37.25
80.01
408.85
Standard deviation
4.07
7.24
22.69
4.53
6.62
38.39
C V (%)
10.12
8.3
6.02
12.16
8.27
9.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IGFBP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IGFBP-1 in samples. No significant cross-reactivity or interference between Rat IGFBP-1 and analogues was observed.
