Rat IGF-1 (Insulin-like Growth Factor 1) ELISA Kit
The Rat IGF-1 (Insulin-like Growth Factor 1) ELISA Kit is a specialized assay intended for the precise and quantitative determination of IGF-1 levels in various biological samples sourced from rats. IGF-1 is a crucial growth factor with pivotal roles in promoting cell growth, proliferation, differentiation, and enhancing overall cellular functions. It plays a significant regulatory role in various biological processes and is essential for tissue development, organ maintenance, and overall growth.
Accurate measurement of IGF-1 levels using this ELISA kit is essential for understanding the regulatory mechanisms governing growth, cellular development, and metabolic functions. Researchers can leverage the data obtained from this assay to gain insights into the involvement of IGF-1 in growth-related disorders, metabolic conditions, and developmental abnormalities. Furthermore, precise quantification of IGF-1 is vital for elucidating its physiological impact and potential therapeutic strategies targeting growth-related pathways. The Rat IGF-1 ELISA Kit by Assay Genie is characterized by exceptional sensitivity and specificity, ensuring dependable and reproducible results. Manufactured under rigorous quality control standards, this kit provides robust performance and user-friendly protocols, making it an optimal choice for researchers investigating growth factors, developmental biology, and metabolic pathways in rat models.
Product Name:
Rat IGF-1 (Insulin-like Growth Factor 1) ELISA Kit
SKU:
AEES00517
Target:
Rat IGF-1 (Insulin-like Growth Factor 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IGF-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IGF-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IGF-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IGF-1. You can calculate the concentration of Rat IGF-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-104
84-97
92-102
Average (%)
101
91
96
1:4
Range (%)
86-98
96-104
96-108
Average (%)
93
99
100
1:8
Range (%)
97-111
94-101
92-101
Average (%)
103
97
95
1:16
Range (%)
94-105
96-105
97-108
Average (%)
102
102
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-108
103
EDTA plasma (n=5)
96-109
102
Cell culture media (n=5)
87-97
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.11
22.37
91.33
8.97
23.14
83.3
Standard deviation
0.54
1.09
4.33
0.47
1.08
4.1
C V (%)
5.93
4.87
4.74
5.24
4.67
4.92
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IGF-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IGF-1 in samples. No significant cross-reactivity or interference between Rat IGF-1 and analogues was observed.
