The Rat IgE (Immunoglobulin E) ELISA Kit is specifically developed to quantitatively measure the levels of Immunoglobulin E (IgE) in a variety of biological samples.
IgE is a type of antibody that plays a crucial role in allergic responses and helps protect against parasite infections. When an individual with a hypersensitivity or allergy is exposed to an allergen, the body produces IgE antibodies that trigger the release of histamine and other mediators, leading to allergic symptoms. Accurate detection and quantification of IgE levels are essential for diagnosing allergies and monitoring immune responses. With Assay Genie's Rat IgE ELISA Kit, researchers can achieve reliable, reproducible, and precise results. This kit is designed with exceptional sensitivity and specificity, ensuring high-quality performance and ease of use, making it an ideal choice for allergy research and immunological studies.
Product Name:
Rat IgE (Immunoglobulin E) ELISA Kit
SKU:
AEES00439
Target:
Rat IgE (Immunoglobulin E)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IgE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IgE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IgE. You can calculate the concentration of Rat IgE in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
91-104
91-102
Average (%)
93
97
97
1:4
Range (%)
94-111
82-94
97-113
Average (%)
102
87
105
1:8
Range (%)
95-111
84-97
96-112
Average (%)
102
91
103
1:16
Range (%)
102-114
87-100
96-109
Average (%)
108
92
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
96
EDTA plasma (n=5)
90-103
96
Cell culture media (n=5)
91-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.8
10.3
45.8
5.3
10.8
47.5
Standard deviation
0.2
0.5
2.3
0.3
0.5
2.4
C V (%)
4.17
4.85
5.02
5.66
4.63
5.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IgE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IgE in samples. No significant cross-reactivity or interference between Rat IgE and analogues was observed.
