The Rat Immunoglobulin A (IgA) ELISA Kit is a specialized assay meticulously designed for the precise and quantitative measurement of Immunoglobulin A levels in various rat biological samples.
Immunoglobulin A is a key component of the immune system, particularly known for its pivotal role in mucosal immunity and defense against pathogens at mucosal surfaces such as the respiratory and gastrointestinal tracts. This ELISA kit allows researchers to accurately determine IgA levels, providing valuable insights into the immune response, mucosal defense mechanisms, and antibody-mediated protection. By offering exceptional sensitivity and specificity, this kit enables detailed analysis of IgA concentrations, crucial for understanding the body's immune defenses and potential alterations in immunological responses. The Rat IgA ELISA Kit by Assay Genie delivers reliable and reproducible results, facilitating in-depth investigations into the dynamic role of Immunoglobulin A in immune surveillance and mucosal immunity. Designed and manufactured to stringent quality control standards, this kit ensures robust and dependable performance while offering ease of use and precision, making it an excellent choice for immunological research and studies focusing on mucosal immune responses.
Product Name:
Rat IgA (Immunoglobulin A) ELISA Kit
SKU:
AEES00530
Target:
Rat IgA (Immunoglobulin A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IgA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IgA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IgA. You can calculate the concentration of Rat IgA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
96-107
87-98
Average (%)
104
104
91
1:4
Range (%)
96-105
96-105
93-104
Average (%)
100
101
99
1:8
Range (%)
88-104
85-97
94-102
Average (%)
96
91
97
1:16
Range (%)
87-96
92-99
92-104
Average (%)
90
96
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
97
EDTA plasma (n=5)
100-109
103
Cell culture media (n=5)
84-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
21.9
57.79
227.3
23.0
58.8
239.04
Standard deviation
1.21
2.73
11.02
1.16
2.83
10.69
C V (%)
5.53
4.72
4.85
5.04
4.81
4.47
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IgA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IgA in samples. No significant cross-reactivity or interference between Rat IgA and analogues was observed.
