The Rat IFNα (Interferon Alpha) ELISA Kit is a specialized assay designed for the quantitative analysis of Interferon Alpha levels in a variety of biological samples collected from rat models. Interferon Alpha is a vital cytokine involved in the regulation of immune responses, particularly in viral infections and autoimmune diseases, serving as a key mediator in antiviral defense mechanisms and immune modulation. This ELISA kit provides researchers with a reliable method to measure and quantify the levels of Interferon Alpha, enabling detailed investigations into its role in immune regulation, antiviral immunity, and inflammatory responses.
Accurate measurement of IFNα is essential for understanding its functions in various disease conditions and immune system activities. Manufactured under strict quality control guidelines, the Rat IFNα ELISA Kit ensures exceptional sensitivity and specificity, allowing for consistent and reproducible results. Its robust performance and user-friendly design make it an excellent choice for research studies focused on immune response modulation and inflammatory processes in rat models.
Product Name:
Rat IFNα (Interferon Alpha) ELISA Kit
SKU:
AEES00549
Target:
Rat IFNα (Interferon Alpha)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IFNα. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IFNα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IFNα, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IFNα. You can calculate the concentration of Rat IFNα in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
92-109
85-100
Average (%)
102
99
91
1:4
Range (%)
95-113
92-106
83-94
Average (%)
103
98
88
1:8
Range (%)
95-110
88-99
85-99
Average (%)
101
94
90
1:16
Range (%)
96-108
93-107
81-94
Average (%)
102
98
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-107
98
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
86-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.13
92.92
447.97
55.02
93.2
486.56
Standard deviation
3.21
3.73
22.8
3.37
4.35
19.95
C V (%)
6.04
4.01
5.09
6.12
4.67
4.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IFNα concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IFNα in samples. No significant cross-reactivity or interference between Rat IFNα and analogues was observed.
