The Rat IFNα (Interferon Alpha) CLIA Kit is a sophisticated assay meticulously designed to quantitatively measure Interferon Alpha levels in rat biological samples. Interferon Alpha, a crucial cytokine in the immune system, plays a pivotal role in modulating antiviral responses, immune regulation, and cellular defenses against pathogens.
Its diverse functions encompass enhancing immune surveillance, activating immune cells, and orchestrating the body's defense mechanisms against viral infections and other immune-related disorders. This state-of-the-art CLIA Kit provides researchers and clinicians with a precise and reliable tool for quantifying IFNα levels, enabling deep insights into immune responses, infectious diseases, and autoimmune conditions. The quantitative assessment of IFNα expression facilitated by this kit allows comprehensive investigations into the role of Interferon Alpha in immune signaling pathways, antiviral immunity, and the modulation of inflammatory responses. With exceptional sensitivity and specificity, the Rat IFNα CLIA Kit ensures accurate and reproducible results, delivering robust performance and high-quality data for a wide range of research applications in immunology, virology, and infectious diseases. Manufactured under stringent quality control standards, this kit offers reliable performance and user-friendly operation, making it an ideal choice for cutting-edge research endeavors and explorations in immunology and immunotherapy.
Product Name:
Rat IFNα (Interferon Alpha) CLIA Kit
SKU:
AEES00732
Target:
Rat IFNα (Interferon Alpha)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IFNα. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IFNα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IFNα, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IFNα. You can calculate the concentration of Rat IFNα in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-110
94-106
95-103
Average (%)
105
101
99
1:4
Range (%)
87-98
87-100
89-99
Average (%)
93
92
92
1:8
Range (%)
90-100
92-104
94-107
Average (%)
97
96
100
1:16
Range (%)
96-108
99-112
92-101
Average (%)
104
105
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-98
94
EDTA plasma (n=5)
99-106
103
Cell culture media (n=5)
86-94
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.94
120.79
432.76
45.45
105.6
453.74
Standard deviation
2.61
5.62
18.44
2.46
5.13
23.82
C V (%)
5.23
4.65
4.26
5.41
4.86
5.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IFNα concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IFNα in samples. No significant cross-reactivity or interference between Rat IFNα and analogues was observed.
