Rat IFN-gamma ELISpot Pair
- SKU:
- RTDC0183
- Product Type:
- ELISpot
- Reactivity:
- Rat
Description
Rat IFN-gamma ELISpot Pair
Assay Genie ELISpot is a highly specific immunoassay for the analysis of IFN-γ production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation. This technique is designed to determine the frequency of IFN-γ producing cells under a given stimulation and the comparison of such frequency against a specific treatment or pathological state. Utilising sandwich immuno-enzyme technology, Assay Genie ELISpot assays can detect both secreted IFN-γ (qualitative analysis) and single cells that produce IFN-γ (quantitative analysis). Cell secreted IFN-γ is captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured IFN-γ is revealed by tracer antibodies and appropriate conjugates.
Rat IFN-gamma ELISpot Pair
The Rat IFN-gamma ELISPOT Pair is a powerful tool for detecting and quantifying interferon-gamma levels in rat samples. This pair includes both capture and detection antibodies, allowing for highly sensitive and specific detection of IFN-gamma in various sample types including serum, plasma, and cell culture supernatants.Interferon-gamma is a key cytokine involved in the immune response, playing a crucial role in inflammation, host defense against pathogens, and immune regulation.
The ability to accurately measure IFN-gamma levels is essential for studying immune responses, evaluating vaccine efficacy, and monitoring immune-related diseases.With its high sensitivity and specificity, the Rat IFN-gamma ELISPOT Pair provides reliable and reproducible results, making it an excellent choice for researchers in the fields of immunology, infectious diseases, and vaccine development. Unlock the potential of your research with this comprehensive ELISPOT pair.
| Product type: | ELISpot Pairs |
| Size: | 10 x 96 Assays |
| Target species: | Rat |
| Specificity: | Recognizes natural rat IFN-γ |
| Incubation: | |
| Kit content: | Assay Genie ELISpot matched antibody pairs are extensively validated and include pre-titrated capture antibody and biotinylated detection antibody. Antibodies are supplied in quantities sufficient for 10 x 96 samples. |
| Synonyms: | IFN-g, IFN-gamma |
| Uniprot: | P01581 |
A capture antibody highly specific for IFN-γ is coated to the wells of a PVDF bottomed 96 well microtitre plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any IFN-γ produced. Cells are then removed by washing prior to the addition of Biotinylated detection antibodies which bind to the previously captured IFN-γ. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing, substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope.
| Step | Procedure |
| 1. | Add 100 µl of PBS 1X to every well. |
| 2. | Incubate plate at room temperature (RT) for 10 min. |
| 2. | Incubate plate at room temperature (RT) for 10 min. |
| 3. | Empty the wells by flicking the plate over a sink & gently tapping on absorbent paper. |
| 3. | Empty the wells by flicking the plate over a sink & gently tapping on absorbent paper. |
| 4. | Add 100 µl of sample, positive and negative controls cell suspension to appropriate wells providing the required concentration of cells and stimulant. |
| 5. | Cover the plate and incubate at 37°C in a CO2 incubator for an appropriate length of time (15-20 hours). Note: do not agitate or move the plate during this incubation. |
| 6. | Empty the wells and remove excess solution then add 100 µl of Wash Buffer to every well. |
| 7. | Incubate the plate at 4°C for 10 min. |
| 8. | Empty the wells as previous and wash the plate 3x with 100 µl of Wash Buffer. |
| 9. | Add 100 µl of diluted detection antibody to every well. |
| 10. | Cover the plate and incubate at RT for 1 hour 30 min. |
| 11. | Empty the wells as previous and wash the plate 3x with 100 µl of Wash Buffer. |
| 12. | Add 100 µl of diluted Streptavidin-AP conjugate to every well. |
| 13. | Cover the plate and incubate at RT for 1 hour. |
| 14. | Empty the wells and wash the plate 3x with 100 µl of Wash Buffer. |
| 15. | Peel of the plate bottom and wash both sides of the membrane 3x under running distilled water, once washing is complete remove any excess solution by repeated tapping on absorbent paper. |
| 16. | Add 100 µl of ready-to-use BCIP/NBT buffer to every well. |
| 17. | Incubate the plate for 5-15 min monitoring spot formation visually throughout the incubation period to assess sufficient colour development. |
| 18. | Empty the wells and rinse both sides of the membrane 3x under running distilled water. Completely remove any excess solution by gentle repeated tapping on absorbent paper. |
| 19. | Read Spots: allow the wells to dry and then read results. The frequency of the resulting coloured spots corresponding to the cytokine producing cells can be determined using an appropriate ELISpot reader and analysis software or manually using a microscope. Note: spots may become sharper after overnight incubation at 4°C in the dark. |
| UniProt Protein Function: | Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. |
| NCBI Summary: | an immune molecule produced by T lymphocytes in response to mitogens or antigens [RGD, Feb 2006] |
| UniProt Code: | P01581 |
| NCBI GenInfo Identifier: | 124482 |
| NCBI Gene ID: | 25712 |
| NCBI Accession: | P01581.1 |
| UniProt Related Accession: | P01581 |
| Molecular Weight: | 17,918 Da |
| NCBI Full Name: | Interferon gamma |
| NCBI Synonym Full Names: | interferon gamma |
| NCBI Official Symbol: | Ifng  |
| NCBI Official Synonym Symbols: | IFNG2  |
| NCBI Protein Information: | interferon gamma; IFN-gamma |
| UniProt Protein Name: | Interferon gamma |
| Protein Family: | Interferon |
| UniProt Gene Name: | Ifng  |
| UniProt Entry Name: | IFNG_RAT |
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