Rat IFABP/FABP2 (Intestinal Fatty Acid Binding Protein) ELISA Kit
The Rat IFABP/FABP2 (Intestinal Fatty Acid Binding Protein) ELISA Kit is a specialized assay designed for the quantitative determination of Intestinal Fatty Acid Binding Protein (IFABP/FABP2) levels in a variety of rat biological samples. FABP2 is known for its crucial role in fatty acid transport and metabolism within the intestines, making it a key player in nutrient uptake and energy regulation processes.
This ELISA kit from Assay Genie provides exceptional sensitivity and specificity to ensure accurate and reproducible results. Manufactured under rigorous quality control standards, this kit offers robust performance and user-friendly protocols, making it an ideal choice for researchers studying the functions and regulatory mechanisms of IFABP/FABP2 in diverse physiological contexts.
Product Name:
Rat IFABP/FABP2 (Intestinal Fatty Acid Binding Protein) ELISA Kit
SKU:
AEES00460
Target:
Rat IFABP/FABP2 (Intestinal Fatty Acid Binding Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IFABP/FABP2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IFABP/FABP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IFABP/FABP2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IFABP/FABP2. You can calculate the concentration of Rat IFABP/FABP2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
89-103
90-103
Average (%)
102
96
97
1:4
Range (%)
94-108
83-95
84-97
Average (%)
99
89
90
1:8
Range (%)
87-102
83-95
87-100
Average (%)
94
89
92
1:16
Range (%)
93-105
86-98
83-94
Average (%)
100
92
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
93
EDTA plasma (n=5)
91-104
97
Cell culture media (n=5)
92-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.39
3.52
0.53
1.31
3.31
Standard deviation
0.03
0.08
0.11
0.03
0.06
0.1
C V (%)
6.12
5.76
3.13
5.66
4.58
3.02
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat IFABP/FABP2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IFABP/FABP2 in samples. No significant cross-reactivity or interference between Rat IFABP/FABP2 and analogues was observed.
