The Rat I-PTH (Intact Parathormone) CLIA Kit is specifically designed for the quantitative measurement of intact parathormone levels in rat serum, plasma, and other biological samples. This kit offers high sensitivity and accuracy, providing reliable and reproducible results for research studies.Intact parathormone is a key hormone that regulates calcium and phosphorus levels in the body, playing a crucial role in bone metabolism and mineral homeostasis. Dysregulation of parathormone levels can lead to various metabolic bone diseases, making it a valuable biomarker for studying bone health and related disorders.
With its user-friendly format and robust performance, the Rat I-PTH CLIA Kit is an essential tool for researchers studying bone metabolism, endocrinology, and related fields. By accurately measuring intact parathormone levels, this kit enables scientists to gain valuable insights into various physiological and pathological processes, paving the way for potential therapeutic interventions.
Product Name:
Rat I-PTH (Intact Parathormone ) CLIA Kit
SKU:
RTES00320
Target:
Rat I-PTH (Intact Parathormone )
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat I-PTH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat I-PTH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat I-PTH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat I-PTH. You can calculate the concentration of Rat I-PTH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
98-111
92-105
Average (%)
91
104
97
1:4
Range (%)
99-113
88-101
86-101
Average (%)
105
95
92
1:8
Range (%)
93-108
90-104
85-97
Average (%)
101
96
92
1:16
Range (%)
89-105
93-104
90-102
Average (%)
96
99
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-115
108
EDTA plasma (n=5)
101-113
107
Cell culture media (n=5)
89-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.13
77.68
171.42
23.48
83.26
187.55
Standard deviation
2.06
5.95
12.65
2.36
9.06
12.47
C V (%)
8.91
7.66
7.38
10.05
10.88
6.65
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat I-PTH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat I-PTH in samples. No significant cross-reactivity or interference between Rat I-PTH and analogues was observed.
