The Rat HSPG (Heparan Sulfate Proteoglycan) ELISA Kit is a sophisticated assay optimized for the precise measurement of Heparan Sulfate Proteoglycan levels in various rat biological samples. HSPGs are essential components of the extracellular matrix and cell surface, crucial for diverse cellular functions such as cell adhesion, migration, signaling, and tissue development. By quantifying HSPG levels, researchers can delve into the intricate roles of these proteoglycans in modulating cellular interactions, tissue homeostasis, and pathological conditions. Given the critical importance of HSPGs in fundamental biological processes, understanding their quantitative levels can provide valuable insights into their involvement in physiological functions, disease progression, and cellular responses to environmental cues and stressors.
The Rat HSPG ELISA Kit offers exceptional sensitivity and specificity, allowing for reliable and reproducible results. Manufactured under stringent quality control measures, this kit ensures robust performance and accuracy, making it an invaluable tool for cutting-edge research into the complex biology of Heparan Sulfate Proteoglycans.
Product Name:
Rat HSPG (Heparan Sulfate Proteoglycan) ELISA Kit
SKU:
AEES00429
Target:
Rat HSPG (Heparan Sulfate Proteoglycan)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HSPG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HSPG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HSPG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HSPG. You can calculate the concentration of Rat HSPG in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-96
95-109
95-109
Average (%)
90
102
103
1:4
Range (%)
90-104
85-99
81-92
Average (%)
96
92
87
1:8
Range (%)
90-104
79-91
86-97
Average (%)
97
86
92
1:16
Range (%)
87-99
81-92
86-100
Average (%)
94
87
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
92
EDTA plasma (n=5)
91-104
96
Cell culture media (n=5)
88-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.99
2.51
9.23
0.97
2.68
8.51
Standard deviation
0.07
0.1
0.41
0.06
0.13
0.29
C V (%)
7.07
3.98
4.44
6.19
4.85
3.41
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat HSPG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat HSPG in samples. No significant cross-reactivity or interference between Rat HSPG and analogues was observed.
