The Rat HSP-90 (Heat Shock Protein 90) ELISA Kit is a cutting-edge tool crafted for the precise quantitative measurement of Heat Shock Protein 90 levels in a variety of rat biological samples. Heat Shock Protein 90, known as HSP-90, is a molecular chaperone critically involved in various cellular processes, including protein folding, assembly, and transport.
As a key regulator of cellular proteostasis, HSP-90 plays a crucial role in maintaining protein homeostasis and ensuring proper cellular function under diverse physiological and stress conditions. This advanced ELISA kit enables researchers to accurately quantify HSP-90 levels, providing valuable insights into the protein's functional significance in cellular health, stress responses, and disease pathogenesis. By measuring the expression of HSP-90, researchers can uncover its roles in protein regulation, cellular signaling, and disease mechanisms, paving the way for the development of novel therapeutic strategies and targeted interventions. Renowned for its exceptional sensitivity and specificity, the Rat HSP-90 ELISA Kit delivers reliable and reproducible results, making it an indispensable tool for research studies investigating the multifaceted functions of HSP-90 in cellular biology and disease processes. With robust performance and user-friendly features, this kit offers researchers a reliable method to explore the intricate roles of HSP-90 in cellular physiology and disease pathophysiology.
Product Name:
Rat HSP-90 (Heat Shock Protein 90) ELISA Kit
SKU:
AEES00426
Target:
Rat HSP-90 (Heat Shock Protein 90)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HSP-90. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HSP-90 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HSP-90, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HSP-90. You can calculate the concentration of Rat HSP-90 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
83-96
86-99
Average (%)
97
90
92
1:4
Range (%)
98-111
85-97
98-111
Average (%)
104
92
104
1:8
Range (%)
99-113
85-96
98-113
Average (%)
107
91
104
1:16
Range (%)
97-112
87-101
94-109
Average (%)
105
93
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
97
EDTA plasma (n=5)
92-107
99
Cell culture media (n=5)
86-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
439.3
709.2
3490.1
453.1
677.9
3471.0
Standard deviation
25.5
37.6
188.5
24.0
39.3
184.0
C V (%)
5.8
5.3
5.4
5.3
5.8
5.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat HSP-90 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat HSP-90 in samples. No significant cross-reactivity or interference between Rat HSP-90 and analogues was observed.
